Enzyme-linked Immunosorbent Assay:Article Title: Antibodies inhibiting c-Met dimerization and uses thereof
Article Snippet: .. To perform these ELISA the human dimeric c-Met protein (R&D sytems, cat# 358MT) is coated at the concentration of 0.7 μg/ml in PBS overnight at 4° C. After saturation of the plates (Costar #3690) with a 0.5% gelatin solution 2 hours at 37° C., hybridoma supernatants are incubated 1 hour at 37° C. Once rinsed with PBS, the anti-mouse HRP-antibody (Jackson ImmunoResearch, catalog #115-035-164) is added to each well at a 1/5000 dilution in ELISA buffer (0.1% gelatin/0.05% Tween 20 in PBS) and the plates incubated for 1 hour at 37° C. After 3 washes in PBS, the activity of the peroxydase is revealed by the addition of 50 μl of TMB substrate (Uptima). ..
Article Title: Anti-cMet antibody and its use for the detection and the diagnosis of cancer
Article Snippet: .. To perform these ELISA the human dimeric c-Met protein (R&D sytems, cat #358MT) is coated at the concentration of 0.25 μg/ml in PBS overnight at 4° C. After saturation of the plates (Costar #3690) with a 0.5% gelatin solution 2 hours at 37° C., hybridoma supernatants are incubated 1 hour at 37° C. Once rinsed with PBS, the anti-mouse HRP-antibody (Jackson ImmunoResearch, catalog #115-035-164) is added to each well at a 1/5000 dilution in ELISA buffer (0.1% gelatin/0.05% Tween 20 in PBS) and the plates incubated for 1 hour at 37° C. After 3 washes in PBS, the activity of the peroxydase is revealed by the addition of 50 μl of TMB substrate (Uptima). ..
Concentration Assay:Article Title: Antibodies inhibiting c-Met dimerization and uses thereof
Article Snippet: .. To perform these ELISA the human dimeric c-Met protein (R&D sytems, cat# 358MT) is coated at the concentration of 0.7 μg/ml in PBS overnight at 4° C. After saturation of the plates (Costar #3690) with a 0.5% gelatin solution 2 hours at 37° C., hybridoma supernatants are incubated 1 hour at 37° C. Once rinsed with PBS, the anti-mouse HRP-antibody (Jackson ImmunoResearch, catalog #115-035-164) is added to each well at a 1/5000 dilution in ELISA buffer (0.1% gelatin/0.05% Tween 20 in PBS) and the plates incubated for 1 hour at 37° C. After 3 washes in PBS, the activity of the peroxydase is revealed by the addition of 50 μl of TMB substrate (Uptima). ..
Article Title: Anti-cMet antibody and its use for the detection and the diagnosis of cancer
Article Snippet: .. To perform these ELISA the human dimeric c-Met protein (R&D sytems, cat #358MT) is coated at the concentration of 0.25 μg/ml in PBS overnight at 4° C. After saturation of the plates (Costar #3690) with a 0.5% gelatin solution 2 hours at 37° C., hybridoma supernatants are incubated 1 hour at 37° C. Once rinsed with PBS, the anti-mouse HRP-antibody (Jackson ImmunoResearch, catalog #115-035-164) is added to each well at a 1/5000 dilution in ELISA buffer (0.1% gelatin/0.05% Tween 20 in PBS) and the plates incubated for 1 hour at 37° C. After 3 washes in PBS, the activity of the peroxydase is revealed by the addition of 50 μl of TMB substrate (Uptima). ..
Incubation:Article Title: Antibodies inhibiting c-Met dimerization and uses thereof
Article Snippet: .. To perform these ELISA the human dimeric c-Met protein (R&D sytems, cat# 358MT) is coated at the concentration of 0.7 μg/ml in PBS overnight at 4° C. After saturation of the plates (Costar #3690) with a 0.5% gelatin solution 2 hours at 37° C., hybridoma supernatants are incubated 1 hour at 37° C. Once rinsed with PBS, the anti-mouse HRP-antibody (Jackson ImmunoResearch, catalog #115-035-164) is added to each well at a 1/5000 dilution in ELISA buffer (0.1% gelatin/0.05% Tween 20 in PBS) and the plates incubated for 1 hour at 37° C. After 3 washes in PBS, the activity of the peroxydase is revealed by the addition of 50 μl of TMB substrate (Uptima). ..
Article Title: Anti-cMet antibody and its use for the detection and the diagnosis of cancer
Article Snippet: .. To perform these ELISA the human dimeric c-Met protein (R&D sytems, cat #358MT) is coated at the concentration of 0.25 μg/ml in PBS overnight at 4° C. After saturation of the plates (Costar #3690) with a 0.5% gelatin solution 2 hours at 37° C., hybridoma supernatants are incubated 1 hour at 37° C. Once rinsed with PBS, the anti-mouse HRP-antibody (Jackson ImmunoResearch, catalog #115-035-164) is added to each well at a 1/5000 dilution in ELISA buffer (0.1% gelatin/0.05% Tween 20 in PBS) and the plates incubated for 1 hour at 37° C. After 3 washes in PBS, the activity of the peroxydase is revealed by the addition of 50 μl of TMB substrate (Uptima). ..
Activity Assay:Article Title: Antibodies inhibiting c-Met dimerization and uses thereof
Article Snippet: .. To perform these ELISA the human dimeric c-Met protein (R&D sytems, cat# 358MT) is coated at the concentration of 0.7 μg/ml in PBS overnight at 4° C. After saturation of the plates (Costar #3690) with a 0.5% gelatin solution 2 hours at 37° C., hybridoma supernatants are incubated 1 hour at 37° C. Once rinsed with PBS, the anti-mouse HRP-antibody (Jackson ImmunoResearch, catalog #115-035-164) is added to each well at a 1/5000 dilution in ELISA buffer (0.1% gelatin/0.05% Tween 20 in PBS) and the plates incubated for 1 hour at 37° C. After 3 washes in PBS, the activity of the peroxydase is revealed by the addition of 50 μl of TMB substrate (Uptima). ..
Article Title: Anti-cMet antibody and its use for the detection and the diagnosis of cancer
Article Snippet: .. To perform these ELISA the human dimeric c-Met protein (R&D sytems, cat #358MT) is coated at the concentration of 0.25 μg/ml in PBS overnight at 4° C. After saturation of the plates (Costar #3690) with a 0.5% gelatin solution 2 hours at 37° C., hybridoma supernatants are incubated 1 hour at 37° C. Once rinsed with PBS, the anti-mouse HRP-antibody (Jackson ImmunoResearch, catalog #115-035-164) is added to each well at a 1/5000 dilution in ELISA buffer (0.1% gelatin/0.05% Tween 20 in PBS) and the plates incubated for 1 hour at 37° C. After 3 washes in PBS, the activity of the peroxydase is revealed by the addition of 50 μl of TMB substrate (Uptima). ..
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